Figure 4.

FoxO1 expression was downregulated by FSH in vitro. Primary MGCs collected from DFs were exposed to 200 μM H₂O₂ for 1 h, rinsed with PBS and cultured in serum-free medium containing 7.5 IU/ml FSH for 12 h. (a) The effects of FSH on FoxO1 expression in MGCs were determined with qRT-PCR. Expression data were normalized to that of β-actin. (b) Western blotting analysis of FoxO1 protein levels in cultured MGCs treated with or without FSH. α-Tubulin was used as the loading control. (c) Quantification of the relative FoxO1 protein level with densitometry. ImageJ 1.42q software was used to analyze the density of each band in Figure 4b, and the relative expression level was normalized to that of α-tubulin. The data represent the mean±S.E. (n=3). *P<0.05; **P<0.01, Student's t-test