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. 2014 Oct 23;5(10):e1479. doi: 10.1038/cddis.2014.430

Figure 5.

Figure 5

Mir-30d upregulates caspase-1 expression through ARC. (a) Relative protein and mRNA levels of ARC in cardiomyocytes treated with different concentrations of glucose (control, 30 and 50 mM). (b) Relative protein and mRNA levels of ARC in the control, mir-30d and NC groups. ARC levels in the HG, HG+AMO-mir-30d and HG+AMO-NC groups in high-glucose-treated cardiomyocytes are shown in the bottom panel. (c) Immunofluorescence images ( × 400) showing the expression of ARC in cardiomyocytes in the control, HG, HG+mir-30d, and HG+AMO-mir-30d groups. Blue: nuclear staining (DAPI); green: ARC staining. (d) Relative protein and mRNA levels of caspase-1 in the control, mir-30d and NC groups. Caspase-1 levels in the HG, HG+AMO-mir-30d and HG+AMO-NC groups in high-glucose-treated cardiomyocytes are shown in the bottom panel. (e) Immunofluorescence images ( × 400) showing expression of caspase-1 in the control, HG, HG+mir-30d, and HG+AMO-mir-30d groups. Blue: nuclear staining (DAPI); green: caspase-1 staining. n=3. *P<0.05 and **P<0.01 versus control; #P<0.05 and ##P<0.01 versus mir-30d; +P<0.05 and ++P<0.01 versus HG; §P <0.05 and §§P<0.01 versus HG+AMO-mir-30d; mean±S.E.M.