Figure 1.

B-ALL cells require glycolysis and express multiple glucose transporters, but Glut1 is limiting for maximal glucose uptake. (a) ECAR was measured in human B-ALL cell lines and primary healthy-donor peripheral blood B cells at baseline and followed by sequential injection of 2 mM glutamine and 25 mM glucose. (b) Viability of human primary B-ALL cells and normal B cells from two donors each over time upon treatment of 2-DG (10 mM). Values were normalized at each time point to the vehicle control for that sample. The BCR-Abl status of primary B-ALL is indicated. (c–e) Control and Glut1^fl/fl CreER B-ALL cells were treated with vehicle or 4-OHT for 96 h followed by 48 h culture without 4-OHT prior to analyses. (c) Glucose transporter family member mRNA expression was measured by qrtPCR. Shown are expression of detected glucose transporters relative to expression of beta-actin. Other Glut family members were not detected. (d) Glut1 protein was measured by immunoblot and (e) glucose uptake was measured with radiolabeled glucose. (f) BCR-Abl activity was analyzed by immunoblot. Means of three or more replicates and S.D. are shown ***P≤0.001, **P≤0.005. NS, not significant