Figure 2.
In vitro Glut1 deletion leads to metabolic reprogramming of B-ALL cells. (a–c) Steady-state metabolite levels in wild-type (WT) Cre-ER and Glut1fl/fl CreER B-ALL cells treated with vehicle or 4-OHT were determined using LC/MS. (a) Principal component, (b) volcano plots of metabolites changed >1.5-fold, P<0.05, and (c) key metabolites categorized by KEGG metabolic pathways are shown in order of significance. (d) Relative levels of specific metabolites in key pathways are shown following Glut1 deletion. Data represent mean and S.D. for triplicate samples. *P<0.05. NS, not significant