Figure 4.
In vitro Glut1 suppresses B-ALL accumulation through decreased proliferation. (a) WT Cre-ER and Glut1fl/fl Cre-ER B-ALL cells were treated with vehicle or 4-OHT for 96 h followed by 48 h of culture without 4-OHT, and cell numbers were counted over time. (b and c) After 4 days treatment with vehicle or 4-OHT followed by 2 days culture without 4-OHT, cells were cultured with BrDU for 1.5 additional hours and (b) BrDU incorporation was measured by intracellular flow cytometry. (c) DNA content was determined flow cytometrically by propidium iodide staining to indicate cell cycle status. Means and S.D. are shown for triplicate samples in representative experiments repeated three or more times. ****P<0.0001. NS, not significant