Figure 2.

GCTB stromal cells express stem cell markers. Proteins from adherent- or spheroidal-growing cells (upper left pictures) were prepared and incubated with the nitrocellulose membranes of an antibody array kit for the detection of human pluripotent stem cell markers. The binding of proteins to antibodies spotted on the membrane was detected using biotinylated secondary antibodies, streptavidin-HRP and chemiluminescence (upper right pictures). The pixel density was quantified using ImageJ software and normalized to the mean pixel intensity of reference spots located at the coordinates A1, A4 and F1 on the membrane. Spot E4 is the negative control, where PBS instead of the antibody was spotted onto the membrane. This experiment was performed with eight different primary cell lines once in duplicate for a general overview and the mean values are shown