Figure 6.

Cabozantinib reduces viability and spheroid and colony formation of GCTB stromal cells. (a) Adherent-growing GCTB stromal cells derived from three different patients were left untreated (CO) or were treated with cabozantinib (10 μM, XL184) or methotrexate (100 μM, MTX). Seventy-two hours later, the viability was measured by the MTT assay, and the control was set to 100%. (b) Spheroidal cultures were established as described in Figure 1b. After spheroid formation, the cells were left untreated or were treated as described above. Seven days later, spheroids were photographed, and the number and volume of spheroids (spheroid surface) were determined. (c) Cells were seeded at a density of 1.5 × 10⁵ cells/ml in six-well plates. After 24 h, the cells were treated as described above. Seventy-two hours later, cells were trypsinized, and 2000 viable cells of each group were seeded per well of a six-well plate. Colony formation was evaluated as described in Figure 1c. Representative pictures of colonies derived from Pat-3 are shown. The data shown are the mean±S.D. (*P<0.05; **P<0.01). Data are representative of three independent experiments with similar results. Data from (a) and (b) are representative of three experiments performed in triplicate (n=9) and data from (c) were performed two times in sextuplicates (n=12)