Figure 6.

Lxn enhances radiation-induced necrosis. (a) Lxn-overexpressing FDC-P1 cells undergo necrosis in response to radiation. TAP-Lxn or TAP-transfected FDC-P1 cells were treated with 6.5 Gy radiation and cell death was determined by Annexin V and 7AAD staining and flow-cytometry analysis. Annexin V-positive and 7AAD-negative cells are apoptotic (labeled as A) and cells positive for both makers are necrotic (labeled as N). Representative flow-cytometry profiles were shown and percentages of apoptotic and necrotic cells at indicated time points post irradiation were labeled. (b) Lxn-overexpressing FDC-P1 cells lack the activation of caspase 3 upon irradiation. Western blot was performed with the antibody against caspase 3 to detect intact and active (cleaved) forms of caspase 3. β-Actin was used as the control