Figure 5.

Overexpression of miR-320c suppressed ALP activity in hMSC. (a) Representative hMSC were stably transduced with a lentivirus containing miR-320c or control miRNA and were subjected to osteoblast differentiation induction for 10 days. ALP staining for control hMSC (LV control) or hMSC stably expressing miR-320c (LV miR-320c) is shown. (b) qRT-PCR analysis of osteoblast gene markers (RUNX2 and osteonectin). (c) Quantification of ALP activity on cells from a. Data is presented as relative ALP activity compared to cells transduced with LV control. Data are presented as mean±S.E. from five independent experiments, n=50 (d) Quantification of cell viability of LV control or LV miR-320c cells on day 10 post-osteogenic induction showing no significant difference between the two groups, *P<0.05, ***P<0.0005