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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Cell Rep. 2014 Feb 27;6(5):783–791. doi: 10.1016/j.celrep.2014.02.003

Figure 3. Cp1-Dependent Cut Isoform Production Required for Dendrite Regrowth.

Figure 3

(A, E, and G) Live imaging of ddaC neuron clones at 95 hr APF, with colorimetric representations of dendritic arbor depth in right panels. (A) Representative cut-mutant ddaC clones are shown. (E) Cp1-mutant ddaC neuron expressing full-length Cut is shown. (G) Cp1-mutant ddaC neuron expressing truncated Cut1176-2207 is shown.

(B–D and F) GFP and HA IHC antibody staining of ddaC neurons (ppk-Gal4; UAS-mCD8::GFP; UAS-cut-HA or UAS-cut1176-2207-HA) during metamorphosis. (B and C) Cut-HA nuclear localization patterns in control background at WP (B) and 24 hr APF (C) are shown. (D) Cut-HA nuclear patterns in Cp1-mutant clones at WP and 24 hr APF are shown. (F) Cut1176-2207-HA nuclear patterns in Cp1-mutant clones at 24 hr APF are shown.

(H) Quantitative analyses of cut-mutant dendrite regrowth defects, cut-mutant rescue experiments with full-length Cut or Cut1176-2207, and Cp1/Cut rescue experiments: field area, branchpoints, depth of primary (1°) and higher-order dendrites from surface, and percentages of total dendrite length at 95 hr APF that are shallow (within 15 μm from the body wall) or deep (≥15 μm) (n ≥ 5 in all groups). *p < 0.001, one-way ANOVA. Error bars represent SEM.

Scale bars, 25 mm (A, E, and G) and 2 mm (B–D and F). See also Figures S3 and S4.