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. 2014 Nov 18;5:5482. doi: 10.1038/ncomms6482

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(a) Schematic diagram of cilium isolation from MDCK monolayers. (b) Visualization of intact cilia in the cilium preparation by immunofluorescence with anti-Ac-tubulin (left). Right panel shows the validation of the cilium preparations by western blot analysis for marker proteins as indicated at right. The cilium preparation (Cilia) and the cell body (CB) without (‘−’) or with (‘+’) tetracycline induction were analysed. (c) Western blot analysis of cilia isolated from non-induced (‘−’) and induced (‘+’) MDCK^PC1WT cells with anti-PC1 or anti-PC2. (d) N-glycosylation analysis of ciliary PC1 and PC2 for the same sample as in (c). Parallel analysis of cell body (CB) served to identify cleavage status of ciliary polycystins. Note that only cleaved and EndoH-resistant PC1 and EndoH-resistant PC2₁₃₀ forms are present in cilia.