Figure 4. MntC-bound plasminogen is converted to functionally active plasmin.

Recombinant proteins or BSA (10 µg/mL), immobilized on microtiter plate wells, were incubated with plasminogen (20 µg/mL). After washing, uPA (3 U) and the chromogenic substrate D-valyl-leucyl-lysine-ρ-nitroanilide dihydrochloride (25 µg/well) were added. Data represent the mean absorbance value at 405 nm ± the standard deviation of two independent experiments, each performed in duplicate. For this analysis, a Student's t-test was used (* p<0.05). PAI-1 (plasminogen activator inhibitor 1).