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. 2014 Nov 19;9(11):e112722. doi: 10.1371/journal.pone.0112722

Figure 4. The expression analysis of selected genes from the expression profile by relative quantitative real-time PCR.

Figure 4

A Transcriptome sequencing data, B Real-time PCR data. Increases and decreases in relative levels of transcripts with respect to the control 18s for mRNA and miR-22a for miRNA are shown. The settings “q.value <0.005” and “|log2.Fold change.normalized|>2” were used as thresholds for judging significant differences in transcript expression. One-way ANOVA tests were performed using SPSS 17.0 to determine significant differences for Real-time PCR data. Statistical significance of the relative expression ratio is indicated *.