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. 2014 Nov 19;9(11):e112564. doi: 10.1371/journal.pone.0112564

Figure 2. EBV induces CD137 expression in T cells.

Figure 2

In vitro EBV infection assay performed in MOLT4 cells. (A) EBNA1 protein expression was examined by immune fluorescence staining, 48 hours after the infection, the time when CD137 expression was examined. (B) Expression of the CD137 gene was examined by RT-PCR. The infection was confirmed by detecting mRNAs of the viral proteins, EBNA1 and LMP1. (C) Transcripts of CD137 and GAPDH were quantified by real time RT-PCR. Relative copy number was obtained by normalizing the CD137 transcripts to those of those of GAPDH. (D) Surface expression of CD137 of MOLT4 cells and EBV-infected MOLT4 cells was examined by flow cytometry.