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. 2014 Nov 19;9(11):e112564. doi: 10.1371/journal.pone.0112564

Figure 3. CD137 expression in EBV-positive T or NK cells of patients with EBV-T/NK-lymphoproliferative disorders (EBV-T/NK-LPDs).

Figure 3

(A) Transcripts of CD137 and GAPDH of freshly isolated EBV-positive cell fractions from 9 EBV-T/NK-LPDs patients, or cells of the same fractions from healthy donors were quantified by real-time RT-PCR. Relative copy number was obtained by normalizing the CD137 transcripts to those of GAPDH. The relative copy number of the EBV-T/NK-LPDs patients' cells and healthy donor cells were compared. (B) CD137 protein expression in peripheral blood mononuclear cells (PBMCs) from 15 EBV-T/NK-LPDs patients or 5 healthy donors. PBMCs were cultured with IL-2 for 3 days and examined by flow cytometry. The mean fluorescent intensity of CD137 was normalized by that of isotype-matched control and expressed as MFIR (mean fluorescence intensity rate). (C) A bar graph for the relative MFIRs. Each point represents the MFIR of each sample.