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. 2014 Nov 19;9(11):e113480. doi: 10.1371/journal.pone.0113480

Figure 4. MaR1 attenuates TNF-α induced inflammatory pathways.

Figure 4

EC were treated with 100 nM MaR1 for 30 min followed by 18 hr of TNF-α (1 ng/ml), after which the conditioned medium was analyzed for the presence of 40 different inflammatory mediators using antibody arrays. (A, B) Representative images of three independent experiments done on EC. (C) After densitometric analysis of individual spots (in duplicate) normalized to protein content (cell lysates), inflammatory mediators that were down-regulated significantly in TNF-α+ MaR1 (t-test: p≤0.05), compared to TNF-α alone are shown in the bar-graph, N = 3. (D) Graphical representation of down-regulated inflammatory proteins found in the media from VSMC that underwent same treatment protocol as (Fig 4 A–C) but with TNF-α at 10 ng/ml. N = 3. *:p≤0.05 compared to TNF-α alone (t-test). N =  each well of a 6-well plate. Error bar  =  SEM.