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. 2014 Oct 30;6(11):1371–1386. doi: 10.15252/emmm.201404033

Figure 2. Development of quality control assays for Ampli1™ WGA.

Figure 2

  1. Representative metaphase CGH experiments for successful (left panel) and failing hybridizations (right panel), which were used to identify the three discriminating amplicons. Table summarizes assay performance for the selected primers.
  2. CTC subcategories according to size and morphology (examples for subcategory I-IV from left to right) are associated with the detection of the QC1 assay amplicons (n = 289, chi-square, P < 0.00001).
  3. Gel picture of the multiplex PCR assay (QC2 assay). Lanes are loaded (left to right): size marker, MU01 CTC04 (GII 0), MU22 CTC01 (GII 2), MU32 CTC01 (GII 2), TB 04 (GII 0), MU28 CTC04 (GII 3), MU35 CTC04 (GII 4), MU12 CTC05 (GII 1), MU28 CTC03 (GII 3), MU35 CTC01 (GII 2), MU28 CTC02 (GII 4), PCR positive control, PCR negative control, size marker.
  4. Summary of sample numbers tested for the different molecular assays.