Table 2.
Correlation between genome integrity index (GII) and successful performance of different molecular assays
| Genomic integrity index (GII) | |||||||
|---|---|---|---|---|---|---|---|
| Molecular assay | Analyzed cells | GII 0 | GII 1 | GII 2 | GII 3 | GII 4 | P-value * |
| PIK3CA Exon 9 | n = 383 | 7 of 23 (30.4%) | 14 of 25 (56.0%) | 48 of 62 (77.4%) | 102 of 117 (87.2%) | 146 of 156 (93.6%) | < 0.00001 |
| PIK3CA Exon 20 | n = 383 | 8 of 23 (34.8%) | 18 of 25 (72.0%) | 55 of 62 (88.7%) | 109 of 117 (93.2%) | 149 of 156 (95.5%) | < 0.00001 |
| PIK3CA complete | n = 383 | 4 of 23 (17.4%) | 12 of 25 (48.0%) | 45 of 62 (72.6%) | 97 of 117 (82.9%) | 141 of 156 (90.4%) | < 0.00001 |
| HER2 qPCR | n = 351 | 3 of 12 (25.0%) | 8 of 18 (50.0%) | 41 of 61 (67.2%) | 95 of 112 (84.8%) | 136 of 148 (91.9%) | < 0.00001 |
| aCGH | n = 50 | Not assessed | Not assessed | 4 of 5 (80.0%) | 7 of 9 (77.8%) | 36 of 36 (100%) | 0.016 |
*
Chi-square test was used to confirm the correlation between performance of molecular assays with genome integrity index. Please note, for raw data on all analyzed single cells see the Supplementary Dataset.