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. 2014 Sep 25;6(11):1387–1397. doi: 10.15252/emmm.201403868

Figure 4. ZIPCO-HA is expressed during late liver stages.

Figure 4

Indirect immunofluorescence assays performed on HepG2 cells 48 h postinfection with WT-F (panels A and D) or ZIPCO-HA sporozoites (Panels B, C and E). Left column, nuclei detected by DAPI labelling shown in white; second column detection of GFP-expressing EEFs (green); third column panel A, B and C: labelling obtained with anti-HA antibody detected with secondary antibody-AF568 shown in red; fourth column labelling of the plasma membrane with anti-MSP1 antibody detected with secondary antibody-AF647 shown in blue. Columns on right show merged images. Panels D and E show the labelling obtained with secondary antibodies alone. Scale bar represents 10 μm.