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. 2014 Sep 30;6(11):1398–1422. doi: 10.15252/emmm.201404168

Figure 3. Immunofluorescence analysis of microglia, macrophages and T cells in the lumbar spinal cord in vehicle and R-flurbiprofen treated C57BL6/J mice (10 mg/kg/day) in the EAE model with preventive treatment (from day 3).

Figure 3

The spinal cord was dissected out during the flare of the disease, day 22. Representative images of 4 mice per group.
  1. Immunofluorescence of ionized calcium binding adaptor molecule 1 (Iba-1, green) in microglia and macrophages. Neurons were counterstained with the neuronal marker NeuN in red. Scale bar 200 μm.
  2. Higher magnification of (A). Scale bar 50 μm.
  3. Immunofluorescence of the T-cell marker CD3 in the white matter of the lumbar spinal cord. Scale bars 50 μm.