Figure 5. Analysis of immune cell infiltration into the spinal cord in naïve and EAE mice after transplantation of bone marrow (BMX) from β-actin-EGFP donor mice.

BMX was performed 3 weeks before immunization. Mice (C57BL6/J) were treated with vehicle or R-flurbiprofen (10 mg/kg/day, drinking water) from the 3rd day after immunization, and tissue was dissected out during the flare of the disease (day 16). BMX treated mice generally develop stronger and earlier EAE.

1. Immunofluorescence of β-actin-EGFP positive infiltrating cells (green). Neurons were counterstained with the neuronal marker NeuN (red). Scale bars 200 μm. Two extreme examples of the R-flurbiprofen treated group with complete and moderate efficacy are shown.
2. Scatter dot plots of the FACS analysis of β-actin-EGFP positive infiltrating T cells (CD4⁺) and myeloid cells (CD11b⁺).
3. Quantitative results of the ‘β-actin low or high’ gate. Myeloid cell infiltrates were significantly reduced in the R-flurbiprofen group. Further quantitative results are given in Supplementary Table S4. The asterisks indicate statistically significant differences versus naïve and as indicated (two-way ANOVA, post hoc Bonferroni for ‘treatment’, n = 5 per group, P-values *¹0.0113, *²0.000962, *³0.0422, *⁴0.0059).