Table 1.
Primers sequences used for the RT-qPCR multiplex assay
| Name | Oligonucleotide sequence (5′–3′) | Working concentration (nmol/L) | Accession number | Position | Amplification size (bp) |
|---|---|---|---|---|---|
| CYP1A2 | TACTTGGAGGCCTTCATC (F) | 400 | NM_000761.3 | 1163–1180 | 130 |
| TTACGAAGACACAGCATTT(R) | 400 | 1274–1292 | |||
| CYP2D6 | ATGAGAACCTGTGCATAG (F) | 400 | NM_000106.4 | 812–829 | 101 |
| CGGATGTAGGATCATGAG (R) | 400 | 895–912 | |||
| CYP3A4 | GGTCCAGTGGGATTTATG (F) | 400 | NM_017460.3 | 429–446 | 82 |
| TTGGAGACAGCAATGATC (R) | 400 | 493–510 | |||
| β-actin | ATCACCATTGGCAATGAG (F) | 400 | NM_001101.3 | 826–843 | 105 |
| GATGGAGTTGAAGGTAGTT(R) | 400 | 912–930 |