Figure 1.

Proliferation of T-cell receptor-transgenic Tregs in vivo after immunization with cognate antigen. Conventional or regulatory T cells were purified from DO11 × rip-mOVA mice, CFSE-labeled, and adoptively transferred into BALB/c recipients. Recipient mice were injected subcutaneously with PBS or OVA emulsified in incomplete Freund's adjuvant. Three days later, the proliferation of DO11⁺ T cells in draining LN was assessed.