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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Glia. 2014 Aug 13;63(1):118–131. doi: 10.1002/glia.22738

Figure 4.

Figure 4

Astroglial function in the presence of LME was assessed by their ability to stimulate greater GDNF mRNA levels by the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA) (Chen et al. 2012). Mixed glial cultures (containing both microglia and astroglia) and highly-enriched astroglial cultures were treated with 1.25 μM of SAHA for 12 hours and changes in GDNF (A) and BDNF (B) mRNA expression levels were assessed. The basal level of GDNF or BDNF mRNA in mixed glia and highly-enriched astroglial cultures is comparable. To emphasize the effect of SAHA treatment, we set vehicle-treated group as 100%. Values were present as mean ± SEM from three independent experiments, with duplicates. ** P <0.001, *** P < 0.0001, Bonferroni t-test compared to basal expression levels of vehicle-treated mixed glia culture.