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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Dev Dyn. 2014 Oct 18;243(12):1606–1618. doi: 10.1002/dvdy.24193

Fig. 3.

Fig. 3

Δldb1-induced activation of early lens, olfactory, and cement gland markers in ectodermal recombinants. A: Schematic of recombinant procedure; Stage 10-11 Δldb1 RNA-injected ectoderm combined with Stage 11-11.5 lineage-labeled ectoderm, cultured to the equivalent of Stages 18-26, processed for in situ hybridization with foxe3, nrl-maf, dlx5, and ag1, and sectioned for analysis. B-C: Expression of foxe3 and lineage labeling of ectoderm, Stage 24. D-E: Expression of nrl-maf and lineage labeling of ectoderm, Stage 26. F-G: Expression of dlx5 and lineage labeling of ectoderm, Stage 18. H-I: Expression of ag1 and lineage labeling of ectoderm, Stage 18. Arrowheads indicate identical locations on each pair of images. Table: Expression of genes in recombinants; markers of the lens, nose, and cement gland are detected in Δldb1-injected recombinants, markers of neural and neural crest tissue are not.