Table 3.
Sequence based prediction of the effect of single amino acid substitutions on ERG11 of Candida albicans
| Sl.no | Mutant | Classifiers | ||||||
|---|---|---|---|---|---|---|---|---|
| Polyphen | Provean | I-MUTANT | MutPred | |||||
| Score | Prediction | Score | Prediction | Effect | DDG value (Kcal/mol) | Probability of deleterious mutation | ||
| 1 | ERG11_D116E | 0.003 (sensitivity: 0.98; specificity: 0.44) | Benign | 0.695 | Neutral | Neutral | -0.47 | 0.672 |
| ERG11_Y118A | 1.000 (sensitivity: 0.00; specificity: 1.00) | Probably Damaging | -9.487 | Deleterious | Large Decrease in stability | -1.83 | 0.764 | |
| 2 | ERG11_P230L | 0.997 (sensitivity: 0.41; specificity: 0.98) | Probably Damaging | -8.773 | Deleterious | Large Decrease in stability | -0.97 | 0.849 |
| 3 | ERG_F380L | 0.831 (sensitivity: 0.95; specificity: 0.82) | Probably Damaging | -3.075 | Deleterious | Large Decrease in stability | -1.28 | 0.583 |
| 4 | ERG11_K342R | 0.005 (sensitivity: 0.97; specificity: 0.74) | Benign | -0.443 | Neutral | Neutral | -0.17 | 0.389 |