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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Anesthesiology. 2014 Dec;121(6):1236–1247. doi: 10.1097/ALN.0000000000000470

Figure 5. TLR2−/− mice have improved leukocyte mitochondrial ΔΨm and intracellular ATP production during severe sepsis.

Figure 5

A-B, Mitochondrial ΔΨm. WT and TLR2−/− mice were subjected to sham or CLP surgical procedures. Twenty-four hours later, the peritoneal cells were harvested, stained with TMRE and analyzed for ΔΨm. A, Representative flow cytometry histograms; B, Combined MFI. The numbers of samples in each group: WT-Sham, n = 5; WT-CLP, n = 12; TLR2KO-Sham, n = 5; TLR2KO-CLP, n = 12. *** P < 0.001 versus sham. ## P < 0.01 versus WT. C, Cellular ATP. Mice were subjected to sham or CLP and 24 h later the peritoneal cells were harvested and analyzed for intracellular ATP level by ATP bioluminescence assay. The numbers of samples in each group: WT-Sham, n = 4, WT-CLP: n = 11, TLR2KO-Sham, n = 5, TLR2KO-CLP, n = 12. *** P < 0.001 versus sham. ## P < 0.01 versus WT. Each error bar represents mean ± SEM. MFI = mean fluorescence intensity. ΔΨm = membrane potential. TMRE = tetramethylrhodamine ethyl ester perchlorate. WT = wild type; TLR2KO = TLR2 knockout; CLP = cecum ligation and puncture; ATP = adenosine triphosphate;