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. Author manuscript; available in PMC: 2015 Jul 1.

Published in final edited form as: Leukemia. 2014 May 14;29(1):218–229. doi: 10.1038/leu.2014.159

CD3⁺CD8⁺CD137⁺ cells from SMM MP-CTL were evaluated for their peptide-specific IFN-γ production or CD107a degranulation in response to individual peptide pulsed K562-A*0201 cells.

A. Representative flow cytometric analyses of MP-CTL generated from one SMM patient demonstrated the peptide-specific IFN-γ production and CD107a degranulation (Q2 gated) by CD3⁺CD8⁺CD137⁺ cells in response to HLA-A2-specific heteroclitic XBP1 US_184-192, heteroclitic XBP1 SP_367-375, native CD138_260-268, and native CS1_239-247 peptide presented by K562-A*0201 cells. Baseline level of IFN-γ production and CD107a degranulation was established in response to no peptide pulsed or irrelevant HLA-A2-specific MAGE-3_271-279 peptide pulsed K562-A*0201 cells.

B. The peptide-specific immune responses were demonstrated in MP-CTL generated from three different SMM patients. The MP-CTL from each individual SMM patient (patient #1, patient #2, patient #3) demonstrated a high level of dual immune activities, % IFN-γ⁺CD107a⁺ cells, within the gated CD3⁺CD8⁺CD137⁺ T cell population, in response to each relevant HLA-A2-specific XBP1 US, XBP1 SP, CD138 or CS1 peptide, presented by K562-A*0201 cells.

CD3⁺CD8⁺CD137⁺ cells from SMM MP-CTL were evaluated for their peptide-specific IFN-γ production or CD107a degranulation in response to individual peptide pulsed K562-A*0201 cells.

A. Representative flow cytometric analyses of MP-CTL generated from one SMM patient demonstrated the peptide-specific IFN-γ production and CD107a degranulation (Q2 gated) by CD3⁺CD8⁺CD137⁺ cells in response to HLA-A2-specific heteroclitic XBP1 US_184-192, heteroclitic XBP1 SP_367-375, native CD138_260-268, and native CS1_239-247 peptide presented by K562-A*0201 cells. Baseline level of IFN-γ production and CD107a degranulation was established in response to no peptide pulsed or irrelevant HLA-A2-specific MAGE-3_271-279 peptide pulsed K562-A*0201 cells.

B. The peptide-specific immune responses were demonstrated in MP-CTL generated from three different SMM patients. The MP-CTL from each individual SMM patient (patient #1, patient #2, patient #3) demonstrated a high level of dual immune activities, % IFN-γ⁺CD107a⁺ cells, within the gated CD3⁺CD8⁺CD137⁺ T cell population, in response to each relevant HLA-A2-specific XBP1 US, XBP1 SP, CD138 or CS1 peptide, presented by K562-A*0201 cells.