Figure 2. CD spectrum of p53(1–93) from 5–85°C.

Panels show A) wild type, B) ALA⁻ C) PRO⁻, and D) ALA⁻PRO⁻. All spectra were measured in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7 using 0.1–0.2 mg/mL of protein and reported in molar residue ellipticity (MRE) units of deg cm² dmol⁻¹ res⁻¹. The data symbols (filled circles) were colored according to the temperature the spectrum was measured using: 5°C (black), 15°C (gray), 25°C (purple), 35°C (blue), 45°C (blue–green), 55°C (green), 65°C (yellow), 75°C (orange), and 85°C (red). The insets show the difference in MRE between the spectra at each temperature relative to the 85°C spectrum.