. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: Proteins. 2014 Oct 10;82(12):3373–3384. doi: 10.1002/prot.24692

Table I. R_h measured for p53(1–93) variants.

R_h values are given in Å. R_h,DSL refers to DLS measured values at 25°C (from Figure 4) and R_h,SEC is room temperature measured values using SEC (from Figure 5B). R_h,DSL and R_h,SEC averaged for each variant is given by R_h,avg. R_h,seq was calculated using the equation R_h = (1.24·f_PRO + 0.904) (0.00759·Q_net + 0.963)·2.49·N^0.509 determined by Marsh and Forman-Kay (52).

	R_h,DSL	K_D	R_h,SEC	R_h,avg	R_h,seq
wild type	32.80 ± 1.25	0.160 ±0.008	31.95	32.4	32.2
ALA⁻	31.08 ± 1.28	0.205 ±0.005	29.65	30.4	32.2
PRO⁻	27.53 ± 1.32	0.249 ±0.005	27.36	27.4	24.3
ALAPRO⁻	27.71 ± 0.92	0.252 ±0.005	27.18	27.4	24.3

Table I. Rh measured for p53(1–93) variants.

Table I. R_h measured for p53(1–93) variants.