Table 1.
Expression vectors and their fusion tags
| Lane | Vector | Fusion tag | BL21 | Rosetta 2 | ||||
|---|---|---|---|---|---|---|---|---|
| 20°C PPB | 37°C PPB | 25°C TBONEX | 20°C PPB | 37°C PPB | 25°C TBONEX | |||
| 1 | pOPINF | 6-His- | 1 | 1 | 1 | 1 | 1 | 1 |
| 2 | pOPINS3C | 6-His-SUMO- | 0.7 | 1.5 | 2.1 | 1.3 | 2.7 | 1.1 |
| 3 | pOPINTRX | 6-His-Thioredoxin- | 1.2 | 1.7 | 4.9 | 1.1 | 0.7 | 0.1 |
| 4 | pOPINMSYB | 6-His-MsyB- | 0.5 | 0.8 | 2.9 | 1.8 | 3.6 | 2.0 |
| 5 | pOPINJ | 6-His-GST- | 0.3 | 1.3 | 0.4 | 0.1 | 0.5 | 0.1 |
| 6 | pOPINHALO7 | 6-His-Halo- | 1.5 | 1.6 | 1.7 | 1.6 | 3.0 | 2.0 |
| 7 | pOPINM | 6-His-MBP- | 0.8 | 1.4 | 2.5 | 0.9 | 1.1 | 0.7 |
| 8 | pOPINTF | 6-His-Trigger Factor- | 0.5 | 1.1 | 1.7 | 0.3 | 0.6 | 0.5 |
| 9 | pOPINNusA | 6-His-NusA- | 0.6 | 1.2 | 1.1 | 0.7 | 0.8 | 0.1 |
Empirical scores of soluble fraction yield in small-scale expression relative to 6-His-construct. Highlighted text in bold within the vector name symbolize the fusion tag.