Table 1.
Mole percentage of fatty acids in H9C2 cell (total cell lysate or mitochondria) after 24 h treatment with 100 μM EPA or 50 μM DHA
| Fatty acid | Total | Mitochondria | ||||
|---|---|---|---|---|---|---|
| (Mol %) | Control | EPA | DHA | Control | EPA | DHA |
| C12:0 | 1.16 ± 0.43 | 1.55 ± 0.32 | 1.58 ± 0.24 | 1.25 ± 0.32 | 1.19 ± 0.25 | 1.76 ± 0.33 |
| C14:0 | 0.60 ± 0.06 | 0.86 ± 0.06 | 1.05 ± 0.07 | 0.98 ± 0.02 | 0.83 ± 0.04 | 0.72 ± 0.05 |
| C15:0 | 0.21 ± 0.06 | 0.22 ± 0.03 | 0.74 ± 0.32 | 0.74 ± 0.19 | 0.75 ± 0.28 | 0.86 ± 0.39 |
| C16:0 | 15.22 ± 2.00 | 15.06 ± 1.98 | 15.89 ± 1.86 | 18.94 ± 1.89 | 15.32 ± 2.11* | 15.56 ± 1.54* |
| C:16:1 t | 5.23 ± 1.18 | 5.16 ± 0.98 | 3.83 ± 1.18 | 5.73 ± 1.18 | 5.63 ± 0.26 | 5.83 ± 0.98 |
| C16:1 | 2.86 ± 0.98 | 1.22 ± 0.81 | 0.97 ± 0.81 | 2.65 ± 0.90 | 2.65 ± 0.60 | 3.45 ± 1.86 |
| C17:0 | 1.19 ± 0.23 | 1.52 ± 0.31 | 1.99 ± 0.23 | 1.45 ± 0.32 | 1.58 ± 0.26 | 2.49 ± 0.23 |
| C18:0 | 21.82 ± 3.72 | 20.15 ± 3.56 | 19.36 ± 3.92 | 22.76 ± 1.72 | 18.73 ± 2.25* | 18.36 ± 3.72* |
| C18:1 | 14.50 ± 1.06 | 12.69 ± 1.04* | 12.50 ± 1.06* | 13.40 ± 1.86 | 9.04 ± 2.02* | 10.95 ± 2.06* |
| C:18:1 n-7 | 6.24 ± 0.98 | 5.06 ± 0.86 | 5.24 ± 0.95 | 5.31 ± 0.99 | 5.78 ± 0.67 | 5.44 ± 1.80 |
| C18:2 t n-6 | 2.35 ± 0.46 | 2.26 ± 0.46 | 2.35 ± 0.84 | 3.86 ± 0.86 | 3.35 ± 0.76 | 3.35 ± 0.46 |
| C18:2 n-6 | 2.97 ± 0.43 | 2.65 ± 0.34 | 2.40 ± 0.43 | 2.28 ± 0.58 | 2.59 ± 0.37 | 2.40 ± 0.73 |
| C18:3 n-3 | 0.27 ± 0.17 | 0.37 ± 0.25 | 1.26 ± 0.87 | 0.17 ± 0.04 | 0.65 ± 0.25 | 0.67 ± 0.27 |
| C20:0 | 0.31 ± 0.32 | 0.56 ± 0.12 | 0.66 ± 0.32 | 0.12 ± 0.02 | 0.18 ± 0.04 | 0.65 ± 0.32 |
| C20:1 | 0.37 ± 0.03 | 0.38 ± 0.04 | 0.15 ± 0.03 | 0.24 ± 0.01 | 0.22 ± 0.02 | 0.87 ± 0.43 |
| C20:2 | 1.79 ± 0.31 | 1.49 ± 0.28 | 1.15 ± 0.35 | 1.12 ± 0.05 | 1.36 ± 0.04 | 1.15 ± 0.41 |
| C21:0 | 0.61 ± 0.26 | 0.51 ± 0.08 | 0.51 ± 0.26 | 1.06 ± 0.02 | 1.52 ± 0.21 | 1.17 ± 0.06 |
| C20:3 n-6 | 1.14 ± 0.26 | 0.97 ± 0.17 | 1.02 ± 0.21 | 0.85 ± 0.18 | 0.93 ± 0.20 | 0.54 ± 0.06 |
| C20:4 n-6 | 8.56 ± 1.15 | 6.67 ± 1.05* | 5.59 ± 1.25* | 7.67 ± 1.15 | 5.04 ± 0.19* | 4.89 ± 0.25* |
| C20:3 n-3 | 0.65 ± 0.15 | 1.22 ± 0.39 | 0.90 ± 0.21 | 0.84 ± 0.19 | 0.70 ± 0.16 | 1.44 ± 0.59 |
| C20:5 n-3 | 0.94 ± 0.25 | 5.52 ± 1.13* | 0.91 ± 0.13 | 0.72 ± 0.13 | 5.52 ± 1.23* | 0.91 ± 0.13 |
| C23:0 | 1.14 ± 0.36 | 0.77 ± 0.56 | 0.99 ± 0.24 | 0.32 ± 0.15 | 0.29 ± 0.12 | 0.54 ± 0.36 |
| C24:0 | 0.91 ± 0.13 | 0.73 ± 0.13 | 0.84 ± 0.25 | 0.58 ± 0.15 | 0.42 ± 0.14 | 0.99 ± 0.43 |
| C22:5 n-3 | 3.18 ± 0.88 | 5.99 ± 1.23* | 3.60 ± 0.48 | 3.05 ± 0.98 | 10.22 ± 2.08* | 3.45 ± 0.96 |
| C22:6 n-3 | 3.45 ± 0.96 | 2.97 ± 0.99 | 13.54 ± 2.89* | 3.90 ± 0.56 | 4.24 ± 0.36 | 10.65 ± 1.23* |
| C24:1 | 1.91 ± 0.12 | 1.70 ± 0.19 | 1.54 ± 0.23 | 0.41 ± 0.11 | 0.28 ± 0.13 | 0.51 ± 0.23 |
| n-3/n-6 PUFA | 0.56 ± 0.08 | 1.35 ± 0.14* | 1.85 ± 0.19*△ | 0.59 ± 0.09 | 1.71 ± 0.18* | 1.35 ± 0.15*△ |
All data are the mean ± S. D. for three separate experiments. PUFA: polyunsaturated fatty acids. *:p <0.05 as compared to the corresponding control; △:p <0.05 compared to the corresponding EPA treatment.