Table 4.

Analysis of energy metabolism of line Slc12a1 ^I299F

	Males		Females
Parameter	Homozygous mutants	Heterozygous mutants	Homozygous mutants	Heterozygous mutants	t -test
Body weight (g)	33.0 ± 0.4	40.0 ± 1.1	31.7 ± 1.3	36.2 ± 1.3	c
Rectal body temperature (°C)	36.2 ± 0.04	36.3 ± 0.1	36.7 ± 0.1	36.6 ± 0.1
Food intake (g/day)	3.7 ± 0.2	4.1 ± 0.1	3.9 ± 0.2	3.9 ± 0.1
Energy uptake (kJ/day)	66.2 ± 4.2	72.6 ± 1.6	69.9 ± 4.0	70.1 ± 2.0
Energy uptake / body weight (kJ/g/day)	2.0 ± 0.1	1.8 ± 0.1	2.2 ± 0.1	2.0 ± 0.1	a
Feces production (g/day)	0.91 ± 0.04	0.91 ± 0.05	0.81 ± 0.08	0.93 ± 0.02
Energy content of feces (kJ/g)	15.3 ± 0.1	15.4 ± 0.1	15.3 ± 0.1	15.2 ± 0.1
Metabolized energy (kJ/day)	51.3 ± 4.2	57.5 ± 1.7	56.4 ± 3.5	54.9 ± 1.7
Metabolized energy / body weight (kJ/g/day)	1.55 ± 0.12	1.45 ± 0.06	1.78 ± 0.09	1.53 ± 0.07
Food assimilation coefficient (%)	77.0 ± 1.7	79.1 ± 1.1	80.7 ± 1.8	78.3 ± 0.4

23-24 week-old mice (n = 7 per genotype and sex) were tested under ad libitum conditions. Data are presented as mean ± standard error of mean. Student’s t-test: homozygous mutants (males and females) vs. heterozygous mutants (males and females) as controls: a, P < 0.05; c, P < 0.001.