Table 5.
Immunology analysis of line Slc12a1 I299F
| |
Males |
Females |
||
|---|---|---|---|---|
| Parameter | Homozygous mutants | Heterozygous mutants | Homozygous mutants | Heterozygous mutants |
| CD3+ |
38.8 ± 1.8 |
35.1 ± 2.3 |
37.4 ± 1.7 |
36.1 ± 1.3 |
| CD4+ |
25.7 ± 1.2 |
22.9 ± 1.6 |
23.3 ± 1.3 |
21.9 ± 1.1 |
| CD8β+ |
22.2 ± 0.9 |
20.6 ± 1.6 |
22.9 ± 1.0 |
22.4 ± 1.0 |
| CD19+ |
28.9 ± 1.1 |
31.1 ± 1.1 |
30.3 ± 1.1 |
29.6 ± 1.0 |
| CD19+CD5+ |
3.5 ± 0.2 |
3.3 ± 0.2 |
3.9 ± 0.2 |
3.5 ± 0.1 |
| CD19+CD5− |
96.4 ± 0.2 |
96.5 ± 0.2 |
95.9 ± 0.2 |
96.3 ± 0.1 |
| CD49b+ |
29.9 ± 1.5b |
23.0 ± 1.6 |
28.8 ± 1.9 |
27.3 ± 1.6 |
| Gr1+ |
31.7 ± 0.8 |
37.0 ± 2.1 |
27.0 ± 1.4 |
30.0 ± 1.3 |
| IgA |
445 ± 14 |
453 ± 40 |
475 ± 18 |
478 ± 17 |
| IgG1 |
130 ± 12 |
158 ± 17 |
174 ± 13 |
211 ± 20 |
| IgG2a |
385 ± 25 |
476 ± 34 |
558 ± 43 |
518 ± 35 |
| IgG2b |
94 ± 4a |
147 ± 21 |
175 ± 10 |
165 ± 8 |
| IgG3 |
324 ± 25a |
424 ± 18 |
465 ± 25 |
408 ± 22 |
| IgM | 1338 ± 142 | 1782 ± 275 | 1623 ± 164 | 1590 ± 195 |
Data are frequencies of main leukocyte subsets in blood (%) and concentration (μg/ml) of antibodies of different isotypes in plasma.
17-week-old mice were tested. No. per genotype and sex: n = 15. Data are presented as mean ± standard error of mean. Student’s t-test vs. heterozygous mutants as controls: aP < 0.05, bP < 0.01.