Figure 2.

TCR-triggered superoxide production is mediated by NOX2 in primary T cells. Splenic T cells from either WT or gp91^phox−/− mice were stimulated with CD3xCD28- or isotype-coated microbeads. Superoxide production was measured with Diogenes assay at 5 min intervals. The values indicate the increase in luminescence in CD3xCD28- relative to isotype-stimulated samples. The data show the mean from 3 independent experiments. 2 WT and 4 gp91^phox−/− mice were used in each experiment.