Figure 6.

NOX2 is not required for primary T-cell activation. (A) Splenic T cells from WT or gp91^phox−/− mice were stimulated with CD3xCD28-coated microbeads. After 16 hours cells were stained with CD25-FITC and CD69-PE mAbs and analyzed by flow cytometry. The data are representative of at least 2 independent experiments. 1 WT and 2 gp91^phox−/− mice were used in each experiment. (B) Quantification of (A). The values indicate the mean fluorescence intensities. (C) Quantification of (A). The values indicate the percentages of CD69⁺CD25⁺ cells.