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. 2014 Aug 19;21(1):76. doi: 10.1186/s12929-014-0076-0

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Copyright © 2014 Chen et al.; licensee BioMed Central

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Interleukin-12 production in peripheral blood mononuclear cells from adults and neonates. Peripheral blood mononuclear cells from adults (n = 8) or neonates (n = 10) were cultured in a 48-well plate (2 × 10⁶ cells/ml) in the presence of 100 ng/ml of lipopolysaccharide. After 24 hours of stimulation, IL-12 production was evaluated in the culture supernatants using enzyme-linked immunosorbent assay. The results, obtained by subtracting the control values from the stimulated values, are expressed in pg/ml.