. 2014 Aug 19;21(1):71. doi: 10.1186/s12929-014-0071-5

Table 2.

Transcript and sequence of each primer used in real time RT-PCR and ChIP assays

Transcript	Sequence
For RT-PCR	Primer
IP-10	F: 5′-AGTTAGCAAGGAAAGGTCT-3′
IP-10	R: 5′-ACATTATAGTGCCAGGT-3′
IL-8	F: 5′-AGATCTGAAGTGTGATGACTCAGG-3′
IL-8	R: 5′-GAAGCTTGTGTGCTCTGCTGTCTC-3′
NKRF	F: 5′-AGAAAGATGGGTTGGACT-3′
NKRF	R: 5′-CTGTGTGGCTCTCGGA-3′
GAPDH	F: 5′-TTCCAGGAGCGAGATCCCT-3′
GAPDH	R: 5′-CACCCATGACGAACATGGG-3′
For ChIP assays	Primer
NKRF promoter,	F: 5′-AGGTTCAAGCAGTTTTCC-3′
NKRF promoter,	R: 5′-CTGTAATCTCAGCACTTTGG-3′
IP-10 promoter	F: 5′-AGGCTGGTCTCAAACT-3′
IP-10 promoter	R: 5′-CCTCCCACATCCAATTACT-3′
IL-8 promoter	F: 5′-GGGCCATCAGTTGCAAATC-3′
IL-8 promoter	R: 5′-TTCCTTCCGGTGGTTTCTTC-3′
IP-10 3′-untranslated region (3′-UTR)	F: 5′- TTGAGTTATAATTACTTAT-3′
IP-10 3′-untranslated region (3′-UTR)	R: 5′-TGAAAAGAAGGGTGAGAAGAG-3′
IL-8 3′-untranslated region (3′-UTR),	F: 5′-AGGTTCAAGCAGTTTTCC-3′
IL-8 3′-untranslated region (3′-UTR),	R: 5′-CTGTAATCTCAGCACTTTGG-3′

F = forward; R = reverse; IP-10 = Interferon gamma induced protein 10 kd; IL-8 = interleukin-8; NKRF = NF-κB repressing factor; ChIP = chromatin immunoprecipitation.