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. 2014 Aug 20;7:62. doi: 10.1186/s13045-014-0062-8

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Copyright © 2014 Qin et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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YM155 suppresses radiation-induced DSB repair in ESCC cells. A. YM155 led to significant increase in γ-H2AX staining. B. YM155 inhibited the formation of radiation-induced Rad51 foci. Eca109 cells were treated with 0.1% DMSO or 10 nM YM155 and then exposed to 4 Gy of X ray. After incubation for the indicated times, the cells were fixed for immunofluorescence detection of γ-H2AX (green) and Rad51 (red) foci. Representative images of nuclei (blue) and foci were shown (left lane), scale bar, 10 μm. Data were expressed as the percentage of cells staining positive for foci and plotted as the mean SEM of 3 independent experiments (right lane). Nuclei containing ≥10 immunoreactive foci were scored as positive for γ-H2AX, and ≥5 foci as positive for RAD51. At least 100 nuclei were counted for each experiment. * and #, P < 0.05; **, P < 0.01.