Table 1.
Binding strength of various saccharides for ConA measured as KI values by FAC. Chromatography was carried out at 5 ℃ with 0.1 M Tris-HCl buffer (pH 7.9) containing 0.1 M NaCl, 1 mM CaCl2, and 1 mM MnCl2
| Compound | KI (mM) |
|---|---|
| D-Glucose | 1.5 |
| D-Mannose | 0.19 |
| N-Acetyl-D-glucosamine | 7.2 |
| Methyl α-D-glucoside | 0.22 |
| Methyl β-D-glucoside | 5.3 |
| Methyl α-D-mannoside | 0.045 |
| p-Nitrophenyl α-D-mannoside | 0.014 |
| Glcα1-1-αGlc | 0.24 |
| Glcα1-2Glc | 0.21 |
| Glcα1-3Glc | 0.34 |
| Glcα1-4Glc | 0.24 |
| Glcα1-6Glc | 0.19 |
| Glcβ1-4Glc | 103 |
| Glcβ1-6Glc | 4.9 |