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. 2014 Aug 5;15(1):85. doi: 10.1186/s12931-014-0085-2

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Copyright © 2014 Veith et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Ingestion of fluorescence-labelledS. aureusNewman and Newmaneapcells by alveolar macrophages over time in wild-type and SP-A^−/−mice (n = 4–10).(A, B) Normal lung histology was observed after the experiment in the SP-A deficient animals (A) and in the wild-type animals (B). (C, D) A representative image of intravital microscopy shows bright, shining phagocytes in the alveoli which have ingested fluorescent bacteria. Clearly less of those phagocytes could be seen in SP-A deficient animals (C) compared to wild-type animals (D). (E-H) The count of such cells is presented in three subsequent intervals (E, F, G). A slight increase was observed over time (H).