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Purification of THF-tagged RNA polymerase II for crystallization
| Purification step | Purity (%)a | Yield (mg) |
|---|---|---|
| Lysate (from 400 g wet cell weight) | <1 | N.D.b |
| Q Sepharose | <1 | N.D. |
| Anti-FLAG M2/Ni Sepharose tandem affinity | 90 | 2.0 |
| Resource Q | >95 | 1.5 |
aAssessed by SDS–polyacrylamide gel electrophoresis (PAGE) and CBB staining
bNot determined
