Figure 4.

Deletion of Dgcr8 causes reduced browning of inguinal WAT. A: Real-time PCR examined the expression of common adipocyte differentiation markers in inguinal WAT from control (CON) and KO mice (n = 6 each group). B: CON and KO mice (6 weeks old) were exposed to 4°C for 48 h, and inguinal WAT total RNAs were isolated to examine BAT-selective marker expression by real-time PCR (n = 6). C: Six-week-old mice were exposed to 4°C for 48 h. Inguinal WAT was isolated for Western blot analysis. Each lane represents a pooled tissue lysate from three individual mice. *P < 0.05, Student t test. Data are shown as means ± SEM.