Figure 5.

MPO deletion attenuates insulin resistance in epididymal WAT and liver. WT and MPO^−/− mice fed the ND or HFD for 16 weeks were intraperitoneally injected with 2 units/kg insulin or saline alone. At 15 minutes after injection, homogenates of epididymal WAT were prepared, and levels of phosphorylated (P)-Akt-S473, P-Akt-T308, and P-IR-β were analyzed by Western blotting (A and C) and quantitated by densitometry (B and D). *P < 0.05 vs. WT control; †P < 0.05 WT vs. insulin-injected MPO^−/−. The expression of P-Akt-S473, P-Akt-T308 (E), P-IR-β, and IR-β (F) in liver homogenates were measured by Western blotting.