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. 2014 Nov 13;63(12):4057–4063. doi: 10.2337/db14-0595

Figure 2.

Figure 2

Loss of liver-derived FGF21, but not adipose-derived FGF21, impairs glucose homeostasis and insulin sensitivity in DIO mice. A–D: DIO WT, FGF21 total knockout (KO), FGF21fl/fl, FGF21 LivKO, and FGF21 AdipoKO mice were fed an HFD for 16 weeks. Levels of hepatic Fgf21 mRNA (A), eWAT Fgf21 mRNA (B), BAT Fgf21 mRNA (C), and plasma FGF21 protein (D) in the indicated mice are shown. E–K: Age-matched male FGF21fl/fl, FGF21 LivKO, and FGF21 AdipoKO mice were individually caged and maintained on chow diet until 10 weeks of age. At 10 weeks of age, all mice were placed on HFD for 10 weeks (n = 8–9/group). FGF21fl/fl control mice were compared only with their knockout littermates. Levels of plasma glucose (E and F) and plasma insulin (G and H) during a GTT (n = 6/group). GTTs were performed by administering 2 g glucose/kg lean body wt i.p. to mice that had been fasted for 16 h. I and J: Plasma glucose levels during an ITT (n = 5–6/group). ITTs were performed by administering 0.75 units insulin/kg body wt i.p. to mice fasted for 4–6 h. K: Oil Red O staining of livers and hematoxylin-eosin staining of eWAT. Data are presented as mean ± SEM. Different lowercase letters represent statistical significance (a, P < 0.05; b, P < 0.01; c, P < 0.005; d, P < 0.001).