Figure 4.

Neural stem cells (NSCs) in SOD1^G93A mice are more susceptible to apoptosis induced by H₂O₂ (flow cytometry).

(A) Representative scatter plots showing apoptosis in H₂O₂-treated NSCs by Annexin V-FITC and PI staining and flow cytometry. (I) Late apoptotic cells; (II) early apoptotic cells; (III) intact cells; (IV) necrotic cells. (B) Quantification of apoptotic cells. NSCs were cultured for 36 hours and then treated with H₂O₂ for 12 hours, followed by apoptosis detection. *P < 0.05; **P < 0.01. The values are the mean ± SD of five independent experiments (one-way analysis of variance followed by the least significant difference post-hoc test). H₂O₂ (–) indicates no treatment. SOD1: Superoxide dismutase 1.