Figure 7.

Effects of adenosine monophosphate-activated protein kinase (AMPK) activator 5-aminoimidazole-4-carboxyamide ribonucleoside (AICAR) and inhibitor compound (CC) on the viability and apoptosis of neural stem cells (NSCs).

(A) Cell viability was significantly affected by the addition of AICAR or compound C. The absorbance value of untreated superoxide dismutase 1 wild-type (SOD1^WT) NSCs was set at 100%. (B) Quantitative assessment of NSC apoptosis by AnnexinV-FITC and PI staining and flow cytometry under different treatment conditions. H₂O₂ (–): Untreated. *P < 0.05; **P < 0.01. Data are presented as the mean ± SD and analyzed using one-way analysis of variance followed by the least significant difference post-hoc test. FITC: Fluoresscen isothiocyante; PI: propidium iodide.