Protein levels of PPARγ and C/EBPα in UCMSCs differentiated in MDI, MDI-I, and MDI-R for 2 and 5 weeks were analyzed via immunoblotting. A: Immunoblots probed for PPARγ and C/EBPα. B and C: Densitometric analysis of PPARγ and C/EBPα levels normalized to α-tubulin. Values are expressed as mean ± SE. Significance between groups was determined using a repeated measures two-way ANOVA, followed by Tukey’s multiple comparisons test. For each time point, * represents a significant difference between MDI and both MDI-I and MDI-R, † represents a significant difference between MDI-I and MDI-R, ‡ represents a significant difference between MDI and MDI-R, and § represents a significant difference between MDI and MDI-I (P < 0.05).