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. 2014 Dec;20(12):1844–1849. doi: 10.1261/rna.047332.114

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© 2014 Standaert et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 1. — Loss of Neat1 leads to lobuloalveolar development and lactation defects. (A) Average weight of offspring from Neat1 heterozygous (+/− mother) and Neat1 KO (−/− mother) female before (3.5 wk) and after weaning (6 wk) and representative images. Statistical significance determined by two-sided t-test. (**) P = 0.0044 at 3.5 wk and (**) P = 0.0002 at 6 wk. (B) Whole-mount hematoxylin staining of wild-type (WT) and Neat1 KO (Neat1^−/−) lactating inguinal mammary glands at 2 d post-parturition. (C) H&E staining on sections obtained from the inguinal mammary glands contralateral to the ones described in B. Comparative quantification of (D) luminal alveolar compartment (K8/18 immunoreactivity); (*) P = 0.028 and (E) cell number (DAPI stain) in the lactating mammary gland of wild-type versus Neat1 KO females; (***) P = 0.0003. Statistical significance determined by two-sided t-test. (F) Immunohistochemical staining of cytokeratin 8/18 on consecutive sections of samples in C. (G) Immunohistochemical staining of β-casein on consecutive sections of samples in C. (H) Comparative quantification of Ki67-positive cells relative to total cell number in mammary glands of pregnant females at 8.5 (*) P = 0.04 and 12.5 (*) P = 0.01 d post-coitum. (I) Immunohistochemical staining of Ki67 on sections obtained from inguinal mammary gland 8.5 d post-coitum of wild-type and Neat1^−/− females.