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. 2014 Dec;20(12):1944–1954. doi: 10.1261/rna.046953.114

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© 2014 Fagan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — A-site binding of ASL^SufJ, ASL^Thr, tRNA^SufJ, and tRNA^Thr. Increasing concentrations of 70S ribosomes were programmed with A-site codons (A) ACC-A, (B) ACC-U, or (C) ACC-C and mixed with 2 nM [³²P] ASL^Thr (□/▵ dashed lines) or ASL^SufJ (▪/▴; solid lines). The presence of the antibiotic paromomycin is indicated by “+”. Similar binding experiments were also performed with tRNA^Thr (□/▵ dashed lines) or tRNA^SufJ (▪/▴; solid lines) with A-site codons (D) ACC-A, (E) ACC-U, or (F) ACC-C in the presence or absence of paromomycin (indicated by “+”).